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Production of cyathane type secondary metabolites by submerged cultures of Hericium erinaceus and evaluation of their antibacterial activity by direct bioautography

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Samples were prepared in lithium dodecyl sulfate (LDS) loading buffer supplemented with sample reducing agent (NuPAGE, Thermo Fisher Scientific, Waltham, USA), heated at 70 C for 10 min, resolved by NuPAGE 412% Bis-Tris gels and LDS-PAGE, and transferred onto a nitrocellulose membrane

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