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Mitochondrial dysfunction in intestinal epithelial cells impairs the ETC, reducing ATP production and inhibiting CBS and -lyase in the transsulfuration pathway, leading to a 3073.8% reduction in cysteine and GSH levels (RBC GSH: 1.78 0.28 mol/g vs

Therefore, integrating patient-specific vasculature is crucial for advancing immune-competent aOoC systems and ensuring reliable disease modelling, particularly in immune-mediated and inflammatory disorders

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its activity could be stimulated by the GSH intracellular levels