glutathione dehydrogenase 1 Overview of glutathione-dependent formaldehyde metabolism. (A) Summary Mitochondrial Glutathione in Cellular Redox

The oxidation of the fusion-constructs was followed by mixing 2 M reduced protein in syringe 1 with variable concentrations of GSSG in syringe 2

When GPX4 activity is lost, or its substrate GSH becomes increasingly depleted, ferroptosis progresses more rapidly

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The similar trend was also observed in the present investigation (Tables 2 and 3)

Based on the assumption that a ternary complex is formed between PfGrx, GSH, and roGFP2 WT (S 2 ), we estimated true k red values from tertiary plots at variable GSH concentrations of 7.3 10 5 M 2 s 1 and 1.3 10 5 M 2 s 1 for PfGrx C88S and PfGrx C32S/C88S , respectively (Fig