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To understand the mechanisms underlying hyperhomocysteinemia in malaria, we looked at the transsulphuration (TS) pathway which involves homocysteine as an important intermediate

This ready-to-use, sterile-filtered liquid formulation is supplemented with Earles Balanced Salt Solution (EBSS), 2 mM L-glutamine, D-glucose (1.0 g/L), and 2.2 g/L sodium bicarbonate (NaHCO3), making it suitable for use in a CO2-controlled incubator atmosphere (typically 5 % CO2)

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The study protocol was approved by the ethics committee of Louisiana State Health Sciences Center-Animal Care Committee (Animal Care protocol number P-15-006), and all the methods were carried out following the approved guidelines