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Sufficient genomic DNA was used for PCR amplification to maintain a representation of 280-511 cells per sgRNA for all baseline and treated cell samples (191-455 cells per sgRNA for reference cell samples)

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In the context of ferroptosis, P53 has been shown to regulate the expression of key genes involved in iron metabolism, lipid peroxidation and antioxidant defense

Nevertheless, our findings demonstrate that cartilage-specific deletion of Igf1 exerts protective effects in two mouse models of osteoarthritis, indicating the important contributing role of cartilage-produced IGF1 in Wnt-triggered joint disease

[Lipopolysaccharide induces astrocyte activation and downregulates the expression of Kir4.1 channel]