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Materials and methods Cell culture and treatments Human 21NT breast cancer cells were cultured in 1 Modified Eagles medium alpha (alpha MEM) (Gibco), 2.8 M hydrocortisone, 1 g/ml insulin, 10% foetal calf serum (FCS), 1% gluta- max, 10 mM HEPES, 0.1 mM NEAA and 12.5 ng/ml Epithelial Growth Factor (EGF)

48 hour fasts are generally safe for healthy adults, according to Fernando

Neurotrophic factors: from molecule to man

The Neuron-Derived Orphan Receptor 1 (NOR1) Is Induced upon Human Alternative Macrophage Polarization and Stimulates the Expression of Markers of the M2 Phenotype

Formal safety data specific to topical AHK-Cu is limited, and theoretical concerns such as copper accumulation with heavy long-term use have not been characterized in human studies