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Role of TLR in B cell development: signaling through TLR4 promotes B cell maturation and is inhibited by TLR2

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After reading an internal communication from the University of Iowa Health Care (UIHC), Semprini was concerned he could no longer trust UIHCs smoking cessation program because of the information they shared about vaping nicotine [2]

Primary antibodies used were tyrosine hydroxylase (Abcam cat# ab112, 1:1000), NeuN (BioLegend cat# 834501, 1:2000), alpha-synuclein (ThermoFisher cat# 32-8100, 1:1000), p-ser129 alpha-synuclein (BioLegend cat# 825702, 1:1000), calbindin D (ThermoFisher cat# MA5-50510, 1:1000), and glutamic acid decarboxylase (GAD) (ThermoFisher Cat# MA5-24909, 1:100)