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The sections were then incubated with Alexa fluor 555-labeled secondary antibody (1:1000 in 1x PBS pH 7.4) (Invitrogen, Thermo Fisher Scientific) for 1 hour at room temperature in a dark, humidified chamber, followed by washing in PBS

7 Current status of clinical trial research Although preclinical studies in cellular and animal models have confirmed the safety and efficacy of MenSC transplantation, the clinical application of MenSCs remains limited compared to other stem cell sources

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