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If you want a less concentrated solution for easier dosing, you could add 2mL of water for a final concentration of 25mg/mL

doi: 10.1074/jbc.M306552200 572 AvalosJLBeverKMWolbergerC

Liposomes can be unilamellar or multilamellar, typically sized between 50500 nm, and their composition and surface properties can be precisely tuned

Cells were incubated at 37 C for 3 days before harvesting

Additionally, according to a prior study, the antioxidant glutathione (GSH) content in cells was lowered and cytosolic peroxide was elevated following MTX treatment (Kesik et al