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Human pancreatic stellate cell (hPSC) culture and treatments HPSCs (Fenghui Biotechnology Co., Ltd., Changsha, China) were cultured in RPMI 1640 medium (Gibco) with glucose (5.5 mmol/L glucose) supplemented with 10% FBS (Gibco) in an incubator with 5% CO2 at 37 C

As previously mentioned mTOR integrates nutrient and hormonal signals to regulate growth and has been described as a major modulator of age-related diseases

No formal Federal Register notice has been published, and no official FDA regulatory rule has been amended to enact this promised reclassification

Cells that survive and grow in vivo but are not capable of growing or dividing by conventional laboratory methods