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The absorbance at 405 nm was monitored at 20 C in a Synergy 2 microtiter plate reader, and the initial slope of the resulting curve was used as a measure of enzyme activity

Specificity constant k cat / K M The k cat reflects nanozyme properties when active sites are saturated with substrate and loses its function at low substrate concentrations 90

These include synthetic pharmaceuticals such as metformin, phenformin or phenobarbital, as well as naturally occurring substances such as resveratrol, berberine [128], and, discussed in this article, CGA [129]

They participate in the metabolism of endogenous compounds, including hormones, fatty acids, and prostaglandins

The bioinformatic analysis was supported by the Medical Research Data Center of Fudan University