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platensis strain LB 2340 from the Texas Algal Culture collection UTEX was grown under constant white light using an alkaline Spirulina medium: solution I (162 mM NaHCO 3 , 38 mM Na 2 CO 3 , and 2.9 mM K 2 HPO 4 in 500 ml dH 2 O) and II (29.4 mM NaNO 3 , 5.74 mM K 2 SO 4 , 17.1 mM NaCl, 0.81 mM MgSO 4 , 0.27 mM CaCl 2 in 500 ml dH 2 O) were autoclaved separately, combined after cooling, and 2 ml of a sterile-filtered 0.1 mM vitamin B 12 solution was added

Shen J, Gilmore EC, Marshall CA, Haddadin M, Reynolds JJ, Eyaid W, Bodell A, Barry B, Gleason D, Allen K, Ganesh VS, Chang BS, Grix A, Hill RS, Topcu M, Caldecott KW, Barkovich AJ, Walsh CA (2010) Mutations in PNKP cause microcephaly, seizures and defects in DNA repair

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C., Thomas, J