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[DOI] [PubMed] [Google Scholar] 41.Hernndez-Hernndez C., Viera-Alcaide I., Morales-Sillero A.M., Fernndez-Bolaos J., Rodrguez-Gutirrez G

These reactions can damage sensitive amino acid residues within the peptide chain, reducing potency and potentially creating degradation products that were not in the original formulation

The Role of the Hexahistidine (His6) Tag The His6 tag is one of the most commonly used tags in recombinant protein purification

Cell lines The human CRC cell lines, CCK-81 and DLD-1, and the gastric cancer cell line, MKN45, were obtained from the Health Science Research Resources Bank (Osaka, Japan)