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The erythrocyte was separated by centrifugation at 800 g for 10 min and the supernatant was used for the estimation of enzymatic antioxidants

In fact, gonadotropin-releasing hormone (GnRH) stimulates gonadotroph cells to produce pulsatile LH and FSH

The mixture was combined with an assay mixture of Amplex Red reagent (20 mM stock to 0.1 mM) and HRP (200 U/ml stock to 0.2 U/ml) and added to the wells at 50 l/well

As with BioID, APEX uses harsh cell lysis conditions, and proteins are recovered via streptavidin affinity purification and identified by MS

GST-Pull Down Technology Introduction to protein interactions The functioning of biological systems is inextricably linked to protein-protein interactions, and important biological processes such as DNA synthesis, gene transcriptional activation, protein translation, modification and localization, and information transfer all involve the role of protein complexes