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CD4 + T cells were isolated from splenocytes using the StemCell CD4 + T cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada)

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While still highly significant versus baseline, the high-dose group actually produced fewer new hairs than the low-dose group consistent with the biphasic dose-response seen in the 2007 Pyo laboratory study where higher copper peptide concentrations inhibited rather than stimulated growth

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