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For neutralization assay, 200 TCID 50 of pseudovirus supernatant was preincubated with serial dilutions of the heat-inactivated plasma samples (see above) for 1 h at 37 C and then added onto ACE2 overexpressing HEK293T cells

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43,44,45,46 Other notable conditions frequently excluded were a history of cancer within 5 years (52%), immunocompromised state (45%), and vascular disease of the lower extremity (42%)

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5 Conclusion and outlook Autophagy and cGAS-STING signaling are two highly connected pathways