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Greco EV, Russo G, Giandalia A, Viazzi F, Pontremoli R, De Cosmo S

It has been suggested by some investigators to circumvent this problem by evaluating the response to an evoked painful visceral stimulus, such as bladder distension, either in normal volunteers, or in subjects with visceral pain

Of note, the commercial Iscoves Modified Dulbeccos Medium (IMDM) and Serum Free Expansion Medium (SFEM), which were used as the basal medium for erythroblast cultures, contain a supraphysiological concentration of cystine at 292 M as compared to physiological plasma concentration of 4075 M cysteine in SCD patients, which decreased further to 3060 M during vaso-occlusive pain episodes 36