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Single-point energies were calculated on the optimized geometries using the B97X-D hybrid functional 87 and the 6-311 + G(2d,p) basis set with ultrafine integration grids for the QM part and the ff14SB, gaff2 and TIP3P force fields for the MM part (protein, substrate and water, respectively) with electrostatic embedding

Mild itching around the injection site as it heals

These findings, although obtained from models of oxidative stress and mitochondrial dysfunction rather than in autism models, are highly relevant because they target biological mechanism that are also characteristic of ASD (Sachdeva et al., 2022)

Sequential Introduction Strategy The most widely recommended approach when combining these peptides is sequential introduction rather than starting both simultaneously