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Jun 2024;42(6):1215-1223.doi:10.55563/clinexprheumatol/3l0ihz

Purification of HaloTag fusion proteins from E

The solvent was removed in a speed vac (5 h, 30 C, Concentrator Plus, Eppendorf), and the resulting dried peptides were stored at 20 C until analysis, described in Binding site identification isoDTB analysis section in the Supplementary Information