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K-562 cells were transfected using the Lipofectamine 2000 Reagent (11,668019, Invitrogen) following the manufacturers instructions

That circulating inflammatory load is what connects a digestive problem to symptoms most people would never associate with the gut, which is why repairing the barrier sometimes improves things that seem unrelated

doi: 10.1056/NEJMoa1406498 10 GaronEBRizviNAHuiRLeighlNBalmanoukianASEderJPet al

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