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Description

To test the inhibitory capacity of different inhibitors in vitro, a fast and quantitative assay for MAPK activity was developed which was based on the concept of phosphorylation assisted luciferase fragment complementation (PhALC) 35

The Chromene derivative, 3,3a-dihydrocyclopenta[ b ]chromen-1(2 H )-one, was reported to cage sulfhydryl within 3 min 29 by ring-opening accelerated Michael addition (Fig