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Cell lysates were incubated for 30 minutes on ice and then centrifuged at 12,000g for 15 minutes at 4 C

Furthermore, the upregulation of intercellular signaling molecules in GY suggests pH regulation during storage

doi: 10.3389/fphar.2025.1592458 Received 12 March 2025 Accepted 07 July 2025 Published 31 July 2025 Volume 16 - 2025 Edited by Marcello Iriti, University of Milan, Italy Reviewed by Quansheng Feng, Chengdu University of Traditional Chinese Medicine, China omali Y El-khawaga, Mansoura University, Egypt Updates Copyright 2025 Wang, Yan, Zhang, Wang, Jiao, Zhu, Tan and Yang