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LC-MS/MS The LC-MS/MS measurements were conducted on a Nexera X2 UHPLC (Shimadzu, Kyoto, Japan) coupled to a triple quadrupole mass spectrometer (LC-MS 8050, Shimadzu, Kyoto, Japan) under positive electrospray ionization (ESI) mode

B12 supplementation plays a critical metabolic role in energy production, methylation, and nervous system function

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Unlike traditional anti-aging compounds that require invasive administration, Syn-AKEs low molecular weight (less than 500 Da) enables effective topical penetration through the stratum corneum, making it particularly valuable for non-invasive cosmetic research applications

For this reason, B12 shots are sometimes used to offset the many symptoms of vitamin B12 deficiency, such as fatigue and weakness, poor moods, and low concentration