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The pixel sizes of recorded images were 0.414 nm and 0.702 nm for the F20 and G20 respectively, and images were processed in Adobe Photoshop

Peptides were assigned to the minimum set of proteins (IPI database entries) that could account for all peptides in the dataset

Enzymatic activity was defined as the quantity of the enzyme required to catalyze the synthesis of 1 nM of the superoxide radical anion per minute

doi: 10.1038/s41598-022-17929-y 223

In the fatigued group as compared with the non-fatigued group, fatty acid metabolism, and glycolysis were relatively enhanced, while in the citric acid cycle, despite higher levels of citrate and isocitrate, succinate was significantly lower, suggesting a blockade in metabolism beyond isocitrate