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Plant Cell 13 , 27932807 (2001)

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Superoxide reporters of the DHE family yield non-specific raw fluorescence, thus quantification must rely on chromatographic separation of DHE2-hydroxyethidium (2-OH-E) by HPLC to measure O 2 and discriminate it from ethidium or other adducts, ideally alongside SOD controls and pathway-selective perturbations (e.g., NOX2 inhibitors, mitochondrial site inhibitors) for source assignment

Nuclear H2AX in the tumor epithelium was scored as negative, low, moderate, or high using a standard scoring system as previously described by us and others (72, 73)