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Additional research is exploring its influence on oxidative stress response, immune modulation, and cellular communication involved in tissue repair and remodeling

Co-immunoprecipitation (Co-IP) assay Cells after indicated treatments were lysed in IP buffer (50 mM Tris-HCl, pH 7.5, 0.5% NP-40 (MF430, Mei5bio), 150 mM NaCl, 5 mM EDTA, 10% Glycerin) with protease inhibitor cocktail (B14001, Bimake) for 30 min at 4 C, followed by the enrichment of target proteins using Flag-M2 beads (B23102, Bimake) or specific antibodies overnight at 4 C

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Hydroxysafflor yellow A exerts beneficial effects by restoring hormone secretion and alleviating oxidative stress in polycystic ovary syndrome mice

With one set of 20 strokes and another set of 10 strokes, the remaining sample was homogenized using a 2-ml homogenizer