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Early chemists discovered that urine from individuals with diabetes contained sugar, which could be detected by tasting the urine or observing fermentation reactions

1 : 100, Abcam, UK) and HDAC4 (ab313474

Additionally, the impact of RCC driver mutations (e.g., VHL, PBRM1, and SETD2 in ccRCC) on ferroptosis pathways is incompletely characterized

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The following primary antibodies had been used: anti-SOD1 (dilution 1:1000, NBP1-47443, Novus Biologicals, Centennial, CO, USA), anti-SOD2 (dilution 1:1000, #13141 Cell Signaling Technology, Danvers, MA, USA), anti-GAPDH (dilution 1:1000, #2118 Cell Signaling Technology, Danvers, MA, USA), anti-OPA1 (dilution 1:1000, #80471 Cell Signaling Technology, Danvers, MA, USA), anti-MFF (dilution 1:1000, #84580 Cell Signaling Technology, Danvers, MA, USA) and anti-vinculin (dilution 1:5000, V9131 Merck KGaA, Darmstadt, Germany)