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Jr Macrophage function in adipose tissue homeostasis and metabolic inflammation

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In accordance with our results, Saito et al

Nat Commun 6:8715

Because some of the dihyrdrolipoyl moiety would spontaneously oxidize to the lipoic moiety in solution, to reduce the disulfide bond before incubation with NO donors, the peptide (50 M in 20 mM sodium phosphate buffer) was reduced using 25 mM tris(2-carboxyethyl)phosphine (70 C for 20 min), and the tris(2-carboxyethyl)phosphine was then removed by stage-tip