CloneTracker™ 5-10 Single Barcode Cell Labeling Kit - 5-10 Unique Constructs + PCR Primers and Reagents CRISPR Cells Description Quantity Provided RSCOV-SPACK SARS-CoV-S

Description Quantity Provided RSCOV-SPACK SARS-CoV-S Pseudotyped Lentiviral Packaging Mix 250 ug RSCOV2-SPACK SARS-CoV2-S Pseudotyped Lentiviral Packaging Mix 250 ug RSCOV2-SDGPACK SARS-CoV2-S-D614G Pseudotyped Lentiviral Packaging Mix 250 ug RSCOV2-SDGB117PACK SARS-CoV2-S-B

BFP) as well as a second construct with a non-target sgRNA and a second fluorescent marker (usually RFP or BFP)

Catalog # Universal Unique Dual-Index (UDI) NGS Plate  Quantity

The design is the same as our dual-sgRNA libraries in the pRSL10-mU6-sgNT1-hU6-sgNT2-UbiC-TagRFP vector

The DriverMap Mouse EXP Targeted Panel Kit includes all the enzymes

CloneTracker™ 5-10 Single Barcode Cell Labeling Kit - 5-10 Unique Constructs + PCR Primers and Reagents CRISPR Cells Description Quantity Provided RSCOV-SPACK SARS-CoV-SChoose 5 or 10 lentiviral constructs with barcodes flanked by common primer sequences to facilitate PCR amplifications. After transduction and antibiotic selection of cells, barcodes can be amplified and sequenced to estimate the number of cells present from a specifically labeled population. In this way, multiple cell populations can be labeled, mixed, growth together, and then the representative numbers of descendant cells from each population can