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1 Introduction Graves disease (GD), an autoimmune thyroid disease (AITD), is the most prevalent cause of hyperthyroidism, potentially resulting in goiter, protruding eyeballs, pretibial mucous edema, and an elevated metabolic state, all of which pose significant health risks ( Research has revealed the importance of critical metals for several typical physiological processes and activities, including copper (Cu), iron (Ir), zinc (Zn), and calcium (Ca)

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