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Intracellular ROS were measured using an ROS detection kit (Invitrogen, CA, USA) following manufacturers protocol, as previously described 31

Psychological stress, infections (especially streptococcal), certain medications (e.g., lithium and beta-blockers), physical skin trauma (Koebner phenomenon), cold and dry climate, and lifestyle factors (obesity, smoking, and excessive alcohol consumption) can all aggravate or start PsO [27]